Self-Sustained Oscillatory Sliding Movement of Doublet Microtubules and Flagellar Bend Formation.

ABSTRACT: It is well established that the basis for flagellar and ciliary movements is ATP-dependent sliding between adjacent doublet microtubules. However, the mechanism for converting microtubule sliding into flagellar and ciliary movements has long remained unresolved. The author has developed new sperm models that use bull spermatozoa divested of their plasma membrane and midpiece mitochondrial sheath by Triton X-100 and dithiothreitol. These models enable the observation of both the oscillatory sliding movement of activated doublet microtubules and flagellar bend formation in the presence of ATP. A long fiber of doublet microtubules extruded by synchronous sliding of the sperm flagella and a short fiber of doublet microtubules extruded by metachronal sliding exhibited spontaneous oscillatory mo

SUBMITTER: Ishijima S 

PROVIDER: S-EPMC4749303 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature