Improved Environmental Genomes via Integration of Metagenomic and Single-Cell Assemblies.

ABSTRACT: Assembling complete or near complete genomes from complex microbial communities remains a significant challenge in metagenomic studies. Recent developments in single cell amplified genomes (SAGs) have enabled the sequencing of individual draft genomes representative of uncultivated microbial populations. SAGs suffer from incomplete and uneven coverage due to artifacts that arise from multiple displacement amplification techniques. Conversely, metagenomic sequence data does not suffer from the same biases as SAGs, and significant improvements have been realized in the recovery of draft genomes from metagenomes. Nevertheless, the inherent genomic complexity of many microbial communities often obfuscates facile generation of population genome assemblies from metagenomic data. Here we describe

SUBMITTER: Mende DR 

PROVIDER: S-EPMC4749706 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature