Comparative proteomics dataset of skimmed milk samples from Holstein and Jersey dairy cattle.

ABSTRACT: Milk samples were collected from Holstein and Jersey breeds of dairy cattle maintained under the same management practices and environmental conditions over a seven-day period. Milk samples were collected twice daily from six cows of each breed as previously described (Tacoma et al., 2016) [1]. Samples were composited within individual cow over the experimental period and skimmed to remove the fat layer. Skimmed milk samples were fractionated using CaCl2 precipitation, ultracentrifugation and ProteoMiner treatment to remove the high abundance milk proteins. Separation of the low abundance proteins was achieved using SDS-PAGE. Differential protein abundances were analyzed by mass spectrometry-based proteomic approaches followed by statistical analyses of the peptide count data. The complete

SUBMITTER: Tacoma R 

PROVIDER: S-EPMC4749939 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature