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Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies.


ABSTRACT: We describe a method to genetically manipulate Chaetomium thermophilum, a eukaryotic thermophile, along with various biochemical applications. The transformation method depends on a thermostable endogenous selection marker operating at high temperatures combined with chromosomal integration of target genes. Our technique allows exploiting eukaryotic thermophiles as source for purifying thermostable native macromolecular complexes with an emphasis on the nuclear pore complex, holding great potential for applications in basic science and biotechnology.

SUBMITTER: Kellner N 

PROVIDER: S-EPMC4750058 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies.

Kellner Nikola N   Schwarz Johannes J   Sturm Miriam M   Fernandez-Martinez Javier J   Griesel Sabine S   Zhang Wenzhu W   Chait Brian T BT   Rout Michael P MP   Kück Ulrich U   Hurt Ed E  

Scientific reports 20160211


We describe a method to genetically manipulate Chaetomium thermophilum, a eukaryotic thermophile, along with various biochemical applications. The transformation method depends on a thermostable endogenous selection marker operating at high temperatures combined with chromosomal integration of target genes. Our technique allows exploiting eukaryotic thermophiles as source for purifying thermostable native macromolecular complexes with an emphasis on the nuclear pore complex, holding great potent  ...[more]

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