Transcriptomic, proteomic and metabolic changes in Arabidopsis thaliana leaves after the onset of illumination.

ABSTRACT:

Background

Light plays an important role in plant growth and development. In this study, the impact of light on physiology of 20-d-old Arabidopsis leaves was examined through transcriptomic, proteomic and metabolomic analysis. Since the energy-generating electron transport chains in chloroplasts and mitochondria are encoded by both nuclear and organellar genomes, sequencing total RNA after removal of ribosomal RNAs provides essential information on transcription of organellar genomes. The changes in the levels of ADP, ATP, NADP(+), NADPH and 41 metabolites upon illumination were also quantified.

Results

Upon illumination, while the transcription of the genes encoded by the plastid genome did not change significantly, the transcription of nuclear genes encoding different func

SUBMITTER: Liang C 

PROVIDER: S-EPMC4750186 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature