Transcriptional analysis of human cranial compartments with different embryonic origins.

ABSTRACT:

Objective

Previous investigations suggest that the embryonic origins of the calvarial tissues (neural crest or mesoderm) may account for the molecular mechanisms underlying sutural development. The aim of this study was to evaluate the differences in the gene expression of human cranial tissues and assess the presence of an expression signature reflecting their embryonic origins.

Methods

Using microarray technology, we investigated global gene expression of cells from the frontal and parietal bones and the metopic and sagittal intrasutural mesenchyme (ISM) of four human foetal calvaria. qRT-PCR of a selected group of genes was done to validate the microarray analysis. Paired comparison and correlation analyses were performed on microarray results.

Results

Of six paire

SUBMITTER: Homayounfar N 

PROVIDER: S-EPMC4750879 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature