Low Dose Iron Treatments Induce a DNA Damage Response in Human Endothelial Cells within Minutes.

ABSTRACT:

Background

Spontaneous reports from patients able to report vascular sequelae in real time, and recognition that serum non transferrin bound iron may reach or exceed 10μmol/L in the blood stream after iron tablets or infusions, led us to hypothesize that conventional iron treatments may provoke acute vascular injury. This prompted us to examine whether a phenotype could be observed in normal human endothelial cells treated with low dose iron.

Methodology

Confluent primary human endothelial cells (EC) were treated with filter-sterilized iron (II) citrate or fresh media for RNA sequencing and validation studies. RNA transcript profiles were evaluated using directional RNA sequencing with no pre-specification of target sequences. Alignments were counted for exons and junctions

SUBMITTER: Mollet IG 

PROVIDER: S-EPMC4750942 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature