Roles of septins in prospore membrane morphogenesis and spore wall assembly in Saccharomyces cerevisiae.

ABSTRACT: The highly conserved family of septin proteins has important functions in cytokinesis in mitotically proliferating cells. A different form of cytokinesis occurs during gametogenesis in Saccharomyces cerevisiae, in which four haploid meiotic products become encased by prospore membrane (PSMs) and specialized, stress-resistant spore walls. Septins are known to localize in a series of structures near the growing PSM, but previous studies noted only mild sporulation defects upon septin mutation. We report that directed PSM extension fails in many septin-mutant cells, and, for those that do succeed, walls are abnormal, leading to increased susceptibility to heating, freezing, and digestion by the Drosophila gut. Septin mutants mislocalize the leading-edge protein (LEP) complex required for norm

SUBMITTER: Heasley LR 

PROVIDER: S-EPMC4751596 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature