Antisocial luxO Mutants Provide a Stationary-Phase Survival Advantage in Vibrio fischeri ES114.

ABSTRACT:

Unlabelled

The squid light organ symbiont Vibrio fischeri controls bioluminescence using two acyl-homoserine lactone pheromone-signaling (PS) systems. The first of these systems to be activated during host colonization, AinS/AinR, produces and responds to N-octanoyl homoserine lactone (C(8)-AHL). We screened activity of a P(ainS)-lacZ transcriptional reporter in a transposon mutant library and found three mutants with decreased reporter activity, low C(8)-AHL output, and other traits consistent with low ainS expression. However, the transposon insertions were unrelated to these phenotypes, and genome resequencing revealed that each mutant had a distinct point mutation in luxO. In the wild type, LuxO is phosphorylated by LuxU and then activates transcription of the small RNA (sRNA)

SUBMITTER: Kimbrough JH 

PROVIDER: S-EPMC4751814 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature