Deriving Heterospecific Self-Assembling Protein-Protein Interactions Using a Computational Interactome Screen.

ABSTRACT: Interactions between naturally occurring proteins are highly specific, with protein-network imbalances associated with numerous diseases. For designed protein-protein interactions (PPIs), required specificity can be notoriously difficult to engineer. To accelerate this process, we have derived peptides that form heterospecific PPIs when combined. This is achieved using software that generates large virtual libraries of peptide sequences and searches within the resulting interactome for preferentially interacting peptides. To demonstrate feasibility, we have (i) generated 1536 peptide sequences based on the parallel dimeric coiled-coil motif and varied residues known to be important for stability and specificity, (ii) screened the 1,180,416 member interactome for predicted Tm values and (ii

SUBMITTER: Crooks RO 

PROVIDER: S-EPMC4751974 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature