Identification of Cell Cycle Dependent Interaction Partners of the Septins by Quantitative Mass Spectrometry.

ABSTRACT: The septins are a conserved family of GTP-binding proteins that, in the baker's yeast, assemble into a highly ordered array of filaments at the mother bud neck. These filaments undergo significant structural rearrangements during the cell cycle. We aimed at identifying key components that are involved in or regulate the transitions of the septins. By combining cell synchronization and quantitative affinity-purification mass-spectrometry, we performed a screen for specific interaction partners of the septins at three distinct stages of the cell cycle. A total of 83 interaction partners of the septins were assigned. Surprisingly, we detected DNA-interacting/nuclear proteins and proteins involved in ribosome biogenesis and protein synthesis predominantly present in alpha-factor arrested that

SUBMITTER: Renz C 

PROVIDER: S-EPMC4752459 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature