Resolving the Complexity of Human Skin Metagenomes Using Single-Molecule Sequencing.

ABSTRACT:

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Deep metagenomic shotgun sequencing has emerged as a powerful tool to interrogate composition and function of complex microbial communities. Computational approaches to assemble genome fragments have been demonstrated to be an effective tool for de novo reconstruction of genomes from these communities. However, the resultant "genomes" are typically fragmented and incomplete due to the limited ability of short-read sequence data to assemble complex or low-coverage regions. Here, we use single-molecule, real-time (SMRT) sequencing to reconstruct a high-quality, closed genome of a previously uncharacterized Corynebacterium simulans and its companion bacteriophage from a skin metagenomic sample. Considerable improvement in assembly quality occurs in hybrid approaches incorpo

SUBMITTER: Tsai YC 

PROVIDER: S-EPMC4752602 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature