A novel human gastric primary cell culture system for modelling Helicobacter pylori infection in vitro.

ABSTRACT:

Background and aims

Helicobacter pylori is the causative agent of gastric diseases and the main risk factor in the development of gastric adenocarcinoma. In vitro studies with this bacterial pathogen largely rely on the use of transformed cell lines as infection model. However, this approach is intrinsically artificial and especially inappropriate when it comes to investigating the mechanisms of cancerogenesis. Moreover, common cell lines are often defective in crucial signalling pathways relevant to infection and cancer. A long-lived primary cell system would be preferable in order to better approximate the human in vivo situation.

Methods

Gastric glands were isolated from healthy human stomach tissue and grown in Matrigel containing media supplemented with various growth f

SUBMITTER: Schlaermann P 

PROVIDER: S-EPMC4752654 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature