A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities.

ABSTRACT:

Background

Cellulose, an abundant and renewable polysaccharides, constitutes the largest resource for bioconversion of biofuels. Plant polysaccharides hydrolysis is catalyzed by cellulases, which include endoglucanases, exoglucanases, and β-glucosidases. Converting cellulose and hemicellulose to short chains of oligosaccharides by endo-/exoglucanases is the key step for biofuel transformation. Intriguingly, β-glucanases with transglycosylation activity not only can relieve product inhibition of glucan hydrolysis but also has potential application as biocatalysts for functional materials.

Results

Here, a metagenomic fosmid library was constructed from a paddy soil for cellulase screening. One purified clone showing carboxymethylcellulase activity was isolated, and the complet

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC4752780 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature