Oufti: an integrated software package for high-accuracy, high-throughput quantitative microscopy analysis.

ABSTRACT: With the realization that bacteria display phenotypic variability among cells and exhibit complex subcellular organization critical for cellular function and behavior, microscopy has re-emerged as a primary tool in bacterial research during the last decade. However, the bottleneck in today's single-cell studies is quantitative image analysis of cells and fluorescent signals. Here, we address current limitations through the development of Oufti, a stand-alone, open-source software package for automated measurements of microbial cells and fluorescence signals from microscopy images. Oufti provides computational solutions for tracking touching cells in confluent samples, handles various cell morphologies, offers algorithms for quantitative analysis of both diffraction and non-diffraction-limi

SUBMITTER: Paintdakhi A 

PROVIDER: S-EPMC4752901 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature