RetFM-J, an ImageJ-based module for automated counting and quantifying features of nuclei in retinal whole-mounts.

ABSTRACT: The present article introduces RetFM-J, a semi-automated ImageJ-based module that detects, counts, and collects quantitative data on nuclei of the inner retina from H&E-stained whole-mounted retinas. To illustrate performance, computer-derived outputs were analyzed in inbred C57BL/6J mice. Automated characterization yielded computer-derived outputs that closely matched manual counts. As a method using open-source software that is freely available, inexpensive staining reagents that are robust, and imaging equipment that is routine to most laboratories, RetFM-J could be utilized in a wide variety of experiments benefiting from high-throughput, quantitative, uniform analyses of total cellularity in the inner retina.

SUBMITTER: Hedberg-Buenz A 

PROVIDER: S-EPMC4753132 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature