Differential expression of pancreatic protein and chemosensing receptor mRNAs in NKCC1-null intestine.

ABSTRACT:

Aim

To investigate the intestinal functions of the NKCC1 Na(+)-K(+)-2Cl cotransporter (SLC12a2 gene), differential mRNA expression changes in NKCC1-null intestine were analyzed.

Methods

Microarray analysis of mRNA from intestines of adult wild-type mice and gene-targeted NKCC1-null mice (n = 6 of each genotype) was performed to identify patterns of differential gene expression changes. Differential expression patterns were further examined by Gene Ontology analysis using the online Gorilla program, and expression changes of selected genes were verified using northern blot analysis and quantitative real time-polymerase chain reaction. Histological staining and immunofluorescence were performed to identify cell types in which upregulated pancreatic digestive enzymes were expre

SUBMITTER: Bradford EM 

PROVIDER: S-EPMC4753180 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature