Genetic Analysis and Detection of fliC H1 and fliC H12 Genes Coding for Serologically Closely Related Flagellar Antigens in Human and Animal Pathogenic Escherichia coli.

ABSTRACT: The E. coli flagellar types H1 and H12 show a high serological cross-reactivity and molecular serotyping appears an advantageous method to establish a clear discrimination between these flagellar types. Analysis of fliC H1 and fliC H12 gene sequences showed that they were 97.5% identical at the nucleotide level. Because of this high degree of homology we developed a two-step real-time PCR detection procedure for reliable discrimination of H1 and H12 flagellar types in E. coli. In the first step, a real-time PCR assay for common detection of both fliC H1 and fliC H12 genes is used, followed in a second step by real-time PCR assays for specific detection of fliC H1 and fliC H12, respectively. The real-time PCR for common detection of fliC H1 and fliC H12 demonstrated 100% sensitivity and spe

SUBMITTER: Beutin L 

PROVIDER: S-EPMC4753304 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature