Comparative proteomic analysis of PAI-1 and TNF-alpha-derived endothelial microparticles.

ABSTRACT: Endothelium-derived microparticles (EMPs) are small vesicles released from endothelial cells in response to cell injury, apoptosis, or activation. Elevated concentrations of EMPs have been associated with many inflammatory and vascular diseases. EMPs also mediate long range signaling and alter downstream cell function. Unfortunately, the molecular and cellular basis of microparticle production and downstream cell function is poorly understood. We hypothesize that EMPs generated by different agonists will produce distinct populations of EMPs with unique protein compositions. To test this hypothesis, different EMP populations were generated from human umbilical vein endothelial cells by stimulation with plasminogen activator inhibitor type 1 (PAI-1) or tumor necrosis factor-alpha (TNF-alpha)

SUBMITTER: Peterson DB 

PROVIDER: S-EPMC4753841 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature