Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.

ABSTRACT: Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV.

SUBMITTER: Ozkumur AY 

PROVIDER: S-EPMC4754792 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature