An advanced bioinformatics approach for analyzing RNA-seq data reveals sigma H-dependent regulation of competence genes in Listeria monocytogenes.

ABSTRACT:

Background

Alternative σ factors are important transcriptional regulators in bacteria. While σ(B) has been shown to control a large regulon and play important roles in stress response and virulence in the pathogen Listeria monocytogenes, the function of σ(H) has not yet been well defined in Listeria, even though σ(H) controls a large regulon in the closely related non-pathogenic Bacillus subtilis.

Results

Using RNA-seq characterization of a L. monocytogenes strain with deletions of all 4 genes encoding alternative σ factors (ΔBCHL), which was further modified to overexpress sigH (ΔBCHL::P rha -sigH), we identified 6 transcription units (TUs) that are transcribed from σ(H)-dependent promoters. Five of these TUs had not been previously identified. Identification of these promo

SUBMITTER: Liu Y 

PROVIDER: S-EPMC4754846 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature