Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencing.

ABSTRACT: West Nile virus (WNV) infection is an emerging mosquito-borne disease that can lead to severe neurological illness and currently has no available treatment or vaccine. Using microengraving, an integrated single-cell analysis method, we analyzed a cohort of subjects infected with WNV - recently infected and post-convalescent subjects - and efficiently identified four novel WNV neutralizing antibodies. We also assessed the humoral response to WNV on a single-cell and repertoire level by integrating next generation sequencing (NGS) into our analysis. The results from single-cell analysis indicate persistence of WNV-specific memory B cells and antibody-secreting cells in post-convalescent subjects. These cells exhibited class-switched antibody isotypes. Furthermore, the results suggest that th

SUBMITTER: Tsioris K 

PROVIDER: S-EPMC4754972 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature