Lessons from ten years of crystallization experiments at the SGC.

ABSTRACT: Although protein crystallization is generally considered more art than science and remains significantly trial-and-error, large-scale data sets hold the promise of providing general learning. Observations are presented here from retrospective analyses of the strategies actively deployed for the extensive crystallization experiments at the Oxford site of the Structural Genomics Consortium (SGC), where comprehensive annotations by SGC scientists were recorded on a customized database infrastructure. The results point to the importance of using redundancy in crystallizing conditions, specifically by varying the mixing ratios of protein sample and precipitant, as well as incubation temperatures. No meaningful difference in performance could be identified between the four most widely used spars

SUBMITTER: Ng JT 

PROVIDER: S-EPMC4756611 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature