In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.

ABSTRACT: Here, a method for presenting crystals of soluble and membrane proteins growing in the lipid cubic or sponge phase for in situ diffraction data collection at cryogenic temperatures is introduced. The method dispenses with the need for the technically demanding and inefficient crystal-harvesting step that is an integral part of the lipid cubic phase or in meso method of growing crystals. Crystals are dispersed in a bolus of mesophase sandwiched between thin plastic windows. The bolus contains tens to hundreds of crystals, visible with an in-line microscope at macromolecular crystallography synchrotron beamlines and suitably disposed for conventional or serial crystallographic data collection. Wells containing the crystal-laden boluses are removed individually from hermetically sealed glass

SUBMITTER: Huang CY 

PROVIDER: S-EPMC4756617 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature