LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex.

ABSTRACT: As obligatory intracellular parasites, viruses rely on cellular machines to complete their life cycle, and most importantly they recruit the host ribosomes to translate their mRNA. The Hepatitis C viral mRNA initiates translation by directly binding the 40S ribosomal subunit in such a way that the initiation codon is correctly positioned in the P site of the ribosome. Such a property is likely to be central for many viruses, therefore the description of host-pathogen interaction at the molecular level is instrumental to provide new therapeutic targets. In this study, we monitored the 40S ribosomal subunit and the viral RNA structural rearrangement induced upon the formation of the binary complex. We further took advantage of an IRES viral mutant mRNA deficient for translation to identify t

SUBMITTER: Angulo J 

PROVIDER: S-EPMC4756818 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature