Attomole quantification and global profile of RNA modifications: Epitranscriptome of human neural stem cells.

ABSTRACT: Exploration of the epitranscriptome requires the development of highly sensitive and accurate technologies in order to elucidate the contributions of the more than 100 RNA modifications to cell processes. A highly sensitive and accurate ultra-high performance liquid chromatography-tandem mass spectrometry method was developed to simultaneously detect and quantify 28 modified and four major nucleosides in less than 20 min. Absolute concentrations were calculated using extinction coefficients of each of the RNA modifications studied. A comprehensive RNA modifications database of UV profiles and extinction coefficient is reported within a 2.3-5.2 % relative standard deviation. Excellent linearity was observed 0.99227-0.99999 and limit of detection values ranged from 63.75 attomoles to 1.21 fe

SUBMITTER: Basanta-Sanchez M 

PROVIDER: S-EPMC4756851 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature