Molecular cloning and expression of Cro s 1: an occupational allergen from saffron pollen (Crocus sativus).

ABSTRACT:

Background

The cultivation of saffron is expanding through the southeast of Iran, and allergy to saffron pollen occurs in workers involved in processing this plant. We aimed to clone, sequence and express a major allergen involved in saffron pollen allergy, and to compare the recombinant with the natural allergen.

Methods

The N-terminal amino acid sequence of Cro s 1, an allergen from saffron pollen, was determined after immunoblotting. The cDNA encoding for this allergen was cloned by PCR utilizing a primer based on the N-terminal amino acid sequence. Recombinant Cro s 1 (rCro s 1) was expressed as a soluble protein in Pichia pastoris and purified to homogeneity by gel filtration. Inhibition of IgE binding to rCro s 1 by pollen extract was analyzed by ELISA.

Section titl

SUBMITTER: Varasteh AR 

PROVIDER: S-EPMC4757073 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature