Optimizing Human Bile Preparation for Two-Dimensional Gel Electrophoresis.

ABSTRACT:

Aims

Bile is an important body fluid which assists in the digestion of fat and excretion of endogenous and exogenous compounds. In the present study, an improved sample preparation for human bile was established.

Methods and material

The method involved acetone precipitation followed by protein extraction using commercially available 2D Clean-Up kit. The effectiveness was evaluated by 2-dimensional electrophoresis (2DE) profiling quality, including number of protein spots and spot distribution.

Results

The total protein of bile fluid in benign biliary disorders was 0.797 ± 0.465 μg/μL. The sample preparation method using acetone precipitation first followed by 2D Clean-Up kit protein extraction resulted in better quality of 2DE gel images in terms of resolution as com

SUBMITTER: Cheng HT 

PROVIDER: S-EPMC4757711 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature