SNP discovery and genetic mapping using genotyping by sequencing of whole genome genomic DNA from a pea RIL population.

ABSTRACT:

Background

Progress in genetics and breeding in pea still suffers from the limited availability of molecular resources. SNP markers that can be identified through affordable sequencing processes, without the need for prior genome reduction or a reference genome to assemble sequencing data would allow the discovery and genetic mapping of thousands of molecular markers. Such an approach could significantly speed up genetic studies and marker assisted breeding for non-model species.

Results

A total of 419,024 SNPs were discovered using HiSeq whole genome sequencing of four pea lines, followed by direct identification of SNP markers without assembly using the discoSnp tool. Subsequent filtering led to the identification of 131,850 highly designable SNPs, polymorphic between at l

SUBMITTER: Boutet G 

PROVIDER: S-EPMC4758021 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature