Synthetically Modified Viral Capsids as Versatile Carriers for Use in Antibody-Based Cell Targeting.

ABSTRACT: The present study describes an efficient and reliable method for the preparation of MS2 viral capsids that are synthetically modified with antibodies using a rapid oxidative coupling strategy. The overall protocol delivers conjugates in high yields and recoveries, requires a minimal excess of antibody to achieve modification of more than 95% of capsids, and can be completed in a short period of time. Antibody-capsid conjugates targeting extracellular receptors on human breast cancer cell lines were prepared and characterized. Notably, conjugation to the capsid did not significantly perturb the binding of the antibodies, as indicated by binding affinities similar to those obtained for the parent antibodies. An array of conjugates was synthesized with various reporters on the interior surfac

SUBMITTER: ElSohly AM 

PROVIDER: S-EPMC4758138 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature