A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters.

ABSTRACT:

Background

Marine mud is an abundant and largely unexplored source of enzymes with unique properties that may be useful for industrial and biotechnological purposes. However, since most microbes cannot be cultured in the laboratory, a cultivation-independent metagenomic approach would be advantageous for the identification of novel enzymes. Therefore, with the objective of screening novel lipolytic enzymes, a metagenomic library was constructed using the total genomic DNA extracted from marine mud.

Results

Based on functional heterologous expression, 34 clones that showed lipolytic activity were isolated. The five clones with the largest halos were identified, and the corresponding genes were successfully overexpressed in Escherichia coli. Molecular analysis revealed that th

SUBMITTER: Gao W 

PROVIDER: S-EPMC4758151 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature