Effect of Hath1 on the proliferation and apoptosis of cutaneous squamous cell carcinoma in vitro.

ABSTRACT: Increasing evidence has demonstrated that the tumor suppressor gene Hath1 is implicated in the development and progression of tumors and is verified to be downregulated in several types of tumor. However, the roles and precise molecular mechanisms of Hath1 in cutaneous squamous cell carcinoma (SCC) remain to be elucidated. In the present study, two approaches were used to investigate the tumor‑suppressing effect of Hath1 in cutaneous SCC. Firstly, the effect of inhibiting Hath1 expression with short hairpin RNA (shRNA) on tumor growth and apoptosis was investigated. KUMA5 cells were stably transfected with a plasmid expressing Hath1 shRNA (pGenesil‑1‑Hath1). Secondly, the anti‑tumor effect of Hath1 was investigated in KUMA5 cells following transfection with pcDNA3.1‑Hath1. The mRNA and pro

SUBMITTER: Ying Z 

PROVIDER: S-EPMC4758284 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature