Differential role of an NF-κB transcriptional response element in endothelial versus intimal cell VCAM-1 expression.

ABSTRACT:

Rationale

Human and murine Vcam1 promoters contain 2 adjacent nuclear factor-κB (NF-κB)-binding elements. Both are essential for cytokine-induced transcription of transiently transfected promoter-reporter constructs. However, the relevance of these insights to regulation of the endogenous Vcam1 gene and to pathophysiological processes in vivo remained unknown.

Objective

Determine the role of the 5' NF-κB-binding element in expression of the endogenous Vcam1 gene.

Methods and results

Homologous recombination in embryonic stem cells was used to inactivate the 5' NF-κB element in the Vcam1 promoter and alter 3 nucleotides in the 5' untranslated region to allow direct comparison of wild-type versus mutant allele RNA expression and chromatin configuration in heterozygous m

SUBMITTER: Milstone DS 

PROVIDER: S-EPMC4758452 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature