Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.

ABSTRACT: The accurate segregation of chromosomes during cell division is achieved by attachment of chromosomes to the mitotic spindle via the kinetochore, a large multi-protein complex that assembles on centromeres. The budding yeast kinetochore comprises more than 60 different proteins. Although the structure and function of many of these proteins has been investigated, we have little understanding of the steady state regulation of kinetochores. The primary model of kinetochore homeostasis suggests that kinetochores assemble hierarchically from the centromeric DNA via the inclusion of a centromere-specific histone into chromatin. We tested this model by trying to perturb kinetochore protein levels by overexpressing an outer kinetochore gene, MTW1. This increase in protein failed to change protein

SUBMITTER: Herrero E 

PROVIDER: S-EPMC4758618 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature