Transcriptomes of lineage-specific Drosophila neuroblasts profiled by genetic targeting and robotic sorting.

ABSTRACT: A brain consists of numerous distinct neurons arising from a limited number of progenitors, called neuroblasts in Drosophila. Each neuroblast produces a specific neuronal lineage. To unravel the transcriptional networks that underlie the development of distinct neuroblast lineages, we marked and isolated lineage-specific neuroblasts for RNA sequencing. We labeled particular neuroblasts throughout neurogenesis by activating a conditional neuroblast driver in specific lineages using various intersection strategies. The targeted neuroblasts were efficiently recovered using a custom-built device for robotic single-cell picking. Transcriptome analysis of mushroom body, antennal lobe and type II neuroblasts compared with non-selective neuroblasts, neurons and glia revealed a rich repertoire of t

SUBMITTER: Yang CP 

PROVIDER: S-EPMC4760311 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature