Metabolic stress is a barrier to Epstein-Barr virus-mediated B-cell immortalization.

ABSTRACT: Epstein-Barr virus (EBV) is an oncogenic herpesvirus that has been causally linked to the development of B-cell and epithelial malignancies. Early after infection, EBV induces a transient period of hyperproliferation that is suppressed by the activation of the DNA damage response and a G1/S-phase growth arrest. This growth arrest prevents long-term outgrowth of the majority of infected cells. We developed a method to isolate and characterize infected cells that arrest after this early burst of proliferation and integrated gene expression and metabolic profiling to gain a better understanding of the pathways that attenuate immortalization. We found that the arrested cells have a reduced level of mitochondrial respiration and a decrease in the expression of genes involved in the TCA cycle an

SUBMITTER: McFadden K 

PROVIDER: S-EPMC4760815 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature