Genome-wide DNA methylation profiling of peripheral blood mononuclear cells in irritable bowel syndrome.

ABSTRACT:

Background

Irritable bowel syndrome (IBS) is a stress-sensitive disorder. Environmental factors including stress can trigger epigenetic changes, which have not been well-studied in IBS. We performed a pilot study investigating genome-wide DNA methylation of IBS patients and healthy controls (HCs) to identify potential epigenetic markers and associated pathways. Additionally, we investigated relationships of epigenetic changes in selected genes with clinical traits.

Methods

Twenty-seven IBS patients (59% women; 10 IBS-diarrhea, 8 IBS-constipation, 9 IBS-mixed) and 23 age- and sex-matched HCs were examined. DNA methylation from peripheral blood mononuclear cells (PBMCs) was measured using HM450 BeadChip, and representative methylation differences were confirmed by bisulphite s

SUBMITTER: Mahurkar S 

PROVIDER: S-EPMC4760882 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature