Involvement of Rac1 signalling pathway in the development and maintenance of acute inflammatory pain induced by bee venom injection.

ABSTRACT:

Background and purpose

The Rho GTPase, Rac1, is involved in the pathogenesis of neuropathic pain induced by malformation of dendritic spines in the spinal dorsal horn (sDH) neurons. In the present study, the contribution of spinal Rac1 to peripheral inflammatory pain was studied.

Experimental approach

Effects of s.c. bee venom (BV) injection on cellular localization of Rac1 in the rat sDH was determined with double labelling immunofluorescence. Activation of Rac1 and its downstream effector p21-activated kinase (PAK), ERKs and p38 MAPK in inflammatory pain states was evaluated with a pull-down assay and Western blotting. The preventive and therapeutic analgesic effects of intrathecal administration of NSC23766, a selective inhibitor of Rac1, on BV-induced spontaneous nocicep

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4761090 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature