Involvement of transglutaminase 2 and voltage-gated potassium channels in cystamine vasodilatation in rat mesenteric small arteries.

ABSTRACT:

Background and purpose

Vasodilatation may contribute to the neuroprotective and vascular anti-remodelling effect of the tissue transglutaminase 2 (TG2) inhibitor cystamine. Here, we hypothesized that inhibition of TG2 followed by blockade of smooth muscle calcium entry and/or inhibition of Rho kinase underlies cystamine vasodilatation.

Experimental approach

We used rat mesenteric small arteries and RT-PCR, immunoblotting, and measurements of isometric wall tension, intracellular Ca(2+) ([Ca(2+)]i ), K(+) currents (patch clamp), and phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and myosin regulatory light chain, in our experiments.

Key results

RT-PCR and immunoblotting revealed expression of TG2 in mesenteric small arteries. Cystamine concentration-

SUBMITTER: Engholm M 

PROVIDER: S-EPMC4761098 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature