The trade-off of availability and growth inhibition through copper for the production of copper-dependent enzymes by Pichia pastoris.

ABSTRACT:

Background

Copper is an essential chemical element for life as it is a part of prosthetic groups of enzymes including super oxide dismutase and cytochrome c oxidase; however, it is also toxic at high concentrations. Here, we present the trade-off of copper availability and growth inhibition of a common host used for copper-dependent protein production, Pichia pastoris.

Results

At copper concentrations ranging from 0.1 mM (6.35 mg/L) to 2 mM (127 mg/L), growth rates of 0.25 h(-1) to 0.16 h(-1) were observed with copper uptake of as high as 20 mgcopper/gCDW. The intracellular copper content was estimated by subtracting the copper adsorbed on the cell wall from the total copper concentration in the biomass. Higher copper concentrations led to stronger cell growth retardation an

SUBMITTER: Balakumaran PA 

PROVIDER: S-EPMC4761204 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature