A novel anti-p21Ras scFv antibody reacting specifically with human tumour cell lines and primary tumour tissues.

ABSTRACT:

Background

The ras genes play an important role in the development and progression of human tumours. Neutralizing Ras proteins in the cytoplasm could be an effective approach to blocking ras signalling. In this study, we prepared anti-p21Ras single chain fragment variable antibody (scFv) and investigated its immunoreactivity with human tumours.

Methods

The coding sequences of H-ras, K-ras, and N-ras were separately ligated into the vector pET-28a(+). Then, recombinant expressing plasmids were induced by IPTG for p21Ras expression in E. coli. Hybridoma cell lines producing anti-p21Ras monoclonal antibodies were isolated using wildtype p21Ras proteins as immunogens. Anti-p21Ras scFv antibody was prepared from the hybridoma by the phage scFv display method. The immunoreactivity

SUBMITTER: Yang JL 

PROVIDER: S-EPMC4761205 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature