Threonine aldolases: perspectives in engineering and screening the enzymes with enhanced substrate and stereo specificities.

ABSTRACT: Threonine aldolases have emerged as a powerful tool for asymmetric carbon-carbon bond formation. These enzymes catalyse the unnatural aldol condensation of different aldehydes and glycine to produce highly valuable β-hydroxy-α-amino acids with complete stereocontrol at the α-carbon and moderate specificity at the β-carbon. A range of microbial threonine aldolases has been recently recombinantly produced by several groups and their biochemical properties were characterized. Numerous studies have been conducted to improve the reaction protocols to enable higher conversions and investigate the substrate scope of enzymes. However, the application of threonine aldolases in organic synthesis is still limited due to often moderate yields and low diastereoselectivities obtained in the aldol reacti

SUBMITTER: Fesko K 

PROVIDER: S-EPMC4761611 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature