C60 fullerene localization and membrane interactions in RAW 264.7 immortalized mouse macrophages.

ABSTRACT: There continues to be a significant increase in the number and complexity of hydrophobic nanomaterials that are engineered for a variety of commercial purposes making human exposure a significant health concern. This study uses a combination of biophysical, biochemical and computational methods to probe potential mechanisms for uptake of C60 nanoparticles into various compartments of living immune cells. Cultures of RAW 264.7 immortalized murine macrophage were used as a canonical model of immune-competent cells that are likely to provide the first line of defense following inhalation. Modes of entry studied were endocytosis/pinocytosis and passive permeation of cellular membranes. The evidence suggests marginal uptake of C60 clusters is achieved through endocytosis/pinocytosis, and that p

SUBMITTER: Russ KA 

PROVIDER: S-EPMC4761875 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature