High-throughput proteomics reveal alarmins as amplifiers of tissue pathology and inflammation after spinal cord injury.

ABSTRACT: Spinal cord injury is characterized by acute cellular and axonal damage followed by aggressive inflammation and pathological tissue remodelling. The biological mediators underlying these processes are still largely unknown. Here we apply an innovative proteomics approach targeting the enriched extracellular proteome after spinal cord injury for the first time. Proteomics revealed multiple matrix proteins not previously associated with injured spinal tissue, including small proteoglycans involved in cell-matrix adhesion and collagen fibrillogenesis. Network analysis of transcriptomics and proteomics datasets uncovered persistent overexpression of extracellular alarmins that can trigger inflammation via pattern recognition receptors. In mechanistic experiments, inhibition of toll-like recept

SUBMITTER: Didangelos A 

PROVIDER: S-EPMC4761922 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature