Complementary Post Transcriptional Regulatory Information is Detected by PUNCH-P and Ribosome Profiling.

ABSTRACT: Two novel approaches were recently suggested for genome-wide identification of protein aspects synthesized at a given time. Ribo-Seq is based on sequencing all the ribosome protected mRNA fragments in a cell, while PUNCH-P is based on mass-spectrometric analysis of only newly synthesized proteins. Here we describe the first Ribo-Seq/PUNCH-P comparison via the analysis of mammalian cells during the cell-cycle for detecting relevant differentially expressed genes between G1 and M phase. Our analyses suggest that the two approaches significantly overlap with each other. However, we demonstrate that there are biologically meaningful proteins/genes that can be detected to be post-transcriptionally regulated during the mammalian cell cycle only by each of the approaches, or their consolidation.

SUBMITTER: Zur H 

PROVIDER: S-EPMC4761937 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature