A novel multiplex bead-based platform highlights the diversity of extracellular vesicles.

ABSTRACT: The surface protein composition of extracellular vesicles (EVs) is related to the originating cell and may play a role in vesicle function. Knowledge of the protein content of individual EVs is still limited because of the technical challenges to analyse small vesicles. Here, we introduce a novel multiplex bead-based platform to investigate up to 39 different surface markers in one sample. The combination of capture antibody beads with fluorescently labelled detection antibodies allows the analysis of EVs that carry surface markers recognized by both antibodies. This new method enables an easy screening of surface markers on populations of EVs. By combining different capture and detection antibodies, additional information on relative expression levels and potential vesicle subpopulations

SUBMITTER: Koliha N 

PROVIDER: S-EPMC4762227 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature