Temporal Phosphoproteome Dynamics Induced by an ATP Synthase Inhibitor Citreoviridin.

ABSTRACT: Citreoviridin, one of toxic mycotoxins derived from fungal species, can suppress lung cancer cell growth by inhibiting the activity of ectopic ATP synthase, but has limited effect on normal cells. However, the mechanism of citreoviridin triggering dynamic molecular responses in cancer cells remains unclear. Here, we performed temporal phosphoproteomics to elucidate the dynamic changes after citreoviridin treatment in cells and xenograft model. We identified a total of 829 phosphoproteins and demonstrated that citreoviridin treatment affects protein folding, cell cycle, and cytoskeleton function. Furthermore, response network constructed by mathematical modeling shows the relationship between the phosphorylated heat shock protein 90 β and mitogen-activated protein kinase signaling pathway.

SUBMITTER: Hu CW 

PROVIDER: S-EPMC4762618 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature