A direct role for cohesin in gene regulation and ecdysone response in Drosophila salivary glands.

ABSTRACT:

Background

Developmental abnormalities observed in Cornelia de Lange syndrome have been genetically linked to mutations in the cohesin machinery. These and other recent experimental findings have led to the suggestion that cohesin, in addition to its canonical function of mediating sister chromatid cohesion, might also be involved in regulating gene expression.

Results

We report that cleavage of cohesin's kleisin subunit in postmitotic Drosophila salivary glands induces major changes in the transcript levels of many genes. Kinetic analyses of changes in transcript levels upon cohesin cleavage reveal that a subset of genes responds to cohesin cleavage within a few hours. In addition, cohesin binds to most of these loci, suggesting that cohesin is directly regulating their exp

SUBMITTER: Pauli A 

PROVIDER: S-EPMC4763543 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature