Myotubes derived from human-induced pluripotent stem cells mirror in vivo insulin resistance.

ABSTRACT: Induced pluripotent stem cells (iPS cells) represent a unique tool for the study of the pathophysiology of human disease, because these cells can be differentiated into multiple cell types in vitro and used to generate patient- and tissue-specific disease models. Given the critical role for skeletal muscle insulin resistance in whole-body glucose metabolism and type 2 diabetes, we have created a novel cellular model of human muscle insulin resistance by differentiating iPS cells from individuals with mutations in the insulin receptor (IR-Mut) into functional myotubes and characterizing their response to insulin in comparison with controls. Morphologically, IR-Mut cells differentiated normally, but had delayed expression of some muscle differentiation-related genes. Most importantly, wherea

SUBMITTER: Iovino S 

PROVIDER: S-EPMC4763759 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature