Molecular Characterization of Transgenic Events Using Next Generation Sequencing Approach.

ABSTRACT: Demand for the commercial use of genetically modified (GM) crops has been increasing in light of the projected growth of world population to nine billion by 2050. A prerequisite of paramount importance for regulatory submissions is the rigorous safety assessment of GM crops. One of the components of safety assessment is molecular characterization at DNA level which helps to determine the copy number, integrity and stability of a transgene; characterize the integration site within a host genome; and confirm the absence of vector DNA. Historically, molecular characterization has been carried out using Southern blot analysis coupled with Sanger sequencing. While this is a robust approach to characterize the transgenic crops, it is both time- and resource-consuming. The emergence of next-gener

SUBMITTER: Guttikonda SK 

PROVIDER: S-EPMC4764375 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature